Unfortunately, this catalyst has a problem with product inhibition.
2.
Activation Mechanism and Regulation through Product Inhibition of the DGC PleD from " C . crescentus"
3.
A proposed mechanism by Lazarus et al . is supported by product inhibition patterns of UDP at saturating peptide conditions.
4.
The failure of the plots to overlay is generally indicative of catalyst activation, deactivation, or product inhibition under the reaction conditions.
5.
Product inhibition is also an important topic in biotechnology, as overcoming this effect can increase the yield of a product, such as an antibiotic.
6.
Product inhibition has long been known to influence catalyst efficiency of many systems, and in the case of same-excess experiments, it prevents the intercepting and parent reactions from overlaying.
7.
Although kinetic studies are hindered by high substrate inhibition, strong product inhibition, very low Km values for the acyl-CoA substrates, and complex detergent effects with respect to micelle formation,
8.
A proposed mechanism supported by product inhibition patterns by UDP at saturating peptide conditions proceeds with starting materials Uridine diphosphate N-acetylglucosamine, and a peptide chain with a reactive serine or threonine hydroxyl group.
9.
These experiments are critical for RPKA of catalytic reactions, as they enable one to probe a number of mechanistic possibilities including catalyst activation ( induction periods ), catalyst deactivation, and product inhibition described in further detail below.
10.
While the failure of the rate vs . substrate concentration plots to overlay under same-excess-same product conditions does not preclude product inhibition, it does, at least, indicate that other catalyst deactivation paths must also be active.
मोबाइल